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Sedimentation methods

The method of successive washes is used to diagnose trematodosis. A sample of faeces (3-5 g) is placed in a glass or a porcelain mortar, poured with a small amount of water and, stirring with a glass rod (pestle in a mortar), add water to 50-100 ml. The mixture is filtered through a sieve or gauze (1 layer) and set aside for 3-5 minutes. Then the upper layer is drained, and the same amount of water is added to the precipitate and the procedure is repeated until the supernatant is clear. The top layer is poured, and the precipitate is poured in portions into Petri dishes or on a glass slide and microscopically.

Modification of the method according to T.G. Nikulin. Considering that with successive pouring off of the supernatant, stirring up occurs, sediment and some of the trematode eggs can be drained. T.G. Nikulin suggested sucking off the supernatant with a pear. When mass studies on the tip of a pear wear a circle of cardboard (limiter). Since the same amount of sediment remains in all the samples in the cups and it does not stir up, the results of the studies are more reliable.

Method of sedimentation with cellophane films according to G.A. Kotelnikov and V.M. Khrenov was suggested for the diagnosis of fascioliasis and dicroceliosis of ruminants, ascariasis and trichocephalosis of pigs. From a 22 µm thick hydrophilic cellophane, rectangular pieces of 2x3 cm are cut and placed in a bacteriological dish with 50% lactic acid or glycerin solution for 24 hours for 24 hours.

After preparing cellophane films, a sample of faeces weighing 2 g is taken and stirred in a glass with a small amount of water. The suspension is filtered through a metal sieve or gauze (1 layer) into a clean glass with a capacity of 50 ml, settled for 15 minutes, the supernatant is poured, 35-40 ml of tap water is added to the precipitate, again defended for 5 minutes, the supernatant is drained glass and covered with cellophane film.
After 8-10 min spend microscopy of the drug. A solution of lactic acid or glycerin clarifies the drug, and the film protects it from drying out.

Flotation-sedimentation method N.V. Demidov used to diagnose fascioliasis. A sample of faeces (from cattle - 5 g, from sheep - 3 g) is placed in a large beaker, filled to the top with a saturated solution of sodium chloride and carefully stirred with a glass rod until a uniform suspension is obtained. The suspension is settled for 15-20 minutes, then the coarse particles floating up on the surface are removed with a teaspoon or shovel. The liquid is sucked off with a pear (at a mass examination merging is allowed), leaving 20-30 ml of it over the sediment, water is added to the sediment to the full volume of the glass and thoroughly stirred with a stick. The suspension is filtered in large glasses, the filtrate is settled for 5 minutes (filtering is done with gauze or a metal sieve with 0.25 mm cells), the liquid from the glasses is sucked off, leaving 15-20 ml of sediment at the bottom. The precipitate is stirred in conical cups, large glasses are rinsed with water and poured, washed into small cups, settled in conical cups for 3-5 minutes, and the liquid is sucked off. This procedure is repeated until the supernatant is clear, then the precipitate is transferred onto a glass slide and microscopically.
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Sedimentation methods

  1. THE METHOD OF "CUTS" AND THE METHOD OF STEP-BY-STEP FORMATION IN THE STUDY OF CHILDREN'S THINKING
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  2. Diagnostic methods
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  3. Diagnostic methods
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  4. GENETIC METHODS
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  5. Research methods
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  7. Acmeological methods
    First of all, I would like to refer to the methods used in acmeology, and, of course, to the actual acmeological methods, because the effectiveness of acmeological research and the efficiency of solving practical problems depend on them. At the early stages of the development of acmeology and to the present, acmeologists were interested in the questions of the competence of applying general scientific methods and methods
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  9. Research methods
    To implement the tasks, a set of scientific methods was used, which were combined in the framework of ascertaining and formative experiments. The main methods: the study of literary sources; Retrospective system-situational analysis of the process of formation of professionalism of teachers; document analysis, questioning, psychological testing, survey, a summary of expert assessments, observation,
  10. TASKS OF THE METHOD
    The genealogical method refers to the most universal methods of genetic analysis. In medicine, it is often referred to as clinical and genealogical, since various diseases or pathological signs are examined. The clinical genealogical method is widely used in solving theoretical problems and applied problems. So resorted to it: 1) when establishing hereditary
  11. DIAGNOSTIC AND PROGNOSTIC VALUE OF THE METHOD
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  12. Research method
    The study of any new field must begin with the search and development of a method. One can express in the form of a general position the idea that any fundamentally new approach to scientific problems inevitably leads to new methods and methods of research. The object and method of research are closely related to each other. Therefore, the study takes a completely different form and flow then
  13. RESEARCH METHODS
    To study the structure of the human body and its functions using various research methods. To study the morphological features of a person, there are two groups of methods. The first group is used to study the structure of the human body on the corpse material, and the second - on a living person. The first group includes: 1) dissection method using simple tools (scalpel, tweezers, saw
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  15. Formula method
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  16. Methods for determining pregnancy in animals
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